Homocysteine (HCY) cystathionine detection reagent

Homocysteine (Hcy) reagent is a reagent used to quantitatively detect total Hcy in human serum and plasma in vitro. This detection reagent is helpful for suspected patients with hyperhomocysteinemia and homocystinemia. Diagnosis and treatment of urinary patients. At present, Hcy has become a recognized cardiovascular disease marker at home and abroad.

Reaction principle: First, the bound or polymerized Hcy (oxidized Hcy) is reduced to free Hcy, and then the free Hcy and serine form cystathionine under the action of cystathionine synthase (CBS), forming Cystathionine forms Hcy, pyruvate and ammonia under the action of cystathionine lyase (CBL). Pyruvate reacts with NADH (coenzyme) under the action of lactate dehydrogenase (LDH). The conversion rate of NADH into NAD+ at 340 nm per minute is directly related to the concentration of Hcy.

Features

1. High specificity and good precision.

2. Liquid dual reagent, fast and simple, suitable for fully automatic biochemical instrument detection.

3. Wide linear range, 0-50μmol/L.

4. Good stability. After 20 days of acceleration at 37°C, there is no significant change in sensitivity; without calibration for 28 days after opening the lid, the deviation is less than 10%.

5. Different ratios of reagents: 4:1, 6:1, 8:1, 10:1, etc.

For use in in vitro diagnostic testing only.