SA detection reagent

Sialic acid is an N- or O-substituted derivative of neuraminic acid, consisting of a monosaccharide and a nine-carbon skeleton. The most common one is N-acetylneuraminic acid. Sialic acid is widely present in animal tissues in the form of glycoproteins and gangliosides, and is also distributed to a certain extent in plants, fungi, and yeast bacteria. Sialic acid levels are related to developmental and pathological stages and are used in clinical medicine for in vitro auxiliary diagnosis of inflammatory diseases and diseases accompanied by tissue destruction, such as malignant tumors.

This reagent is used for the in vitro quantitative determination of sialic acid content in human serum, and has clinical significance for the early diagnosis and prognosis observation of malignant tumors.

Principle: SA in serum is affected by neuraminidase to form N-acetylneuraminic acid, which then generates pyruvate and N-acetylneuraminic acid under the action of N-acetylneuraminic acid aldolase (NANA-aldolase). Acetylmannosamine; pyruvate generates H2O2 under the action of pyruvate oxidase. With the help of Trinder reaction, colored quinone is generated under the action of POD, causing an increase in absorbance at a wavelength of 540nm. By measuring the absorbance change at a wavelength of 540nm and comparing it with the calibrator that has undergone the same treatment, the SA content in the sample can be calculated.

Sample volume: 8μL; R1 dosage: 240μL; R2 dosage: 80μL

Main wavelength: 340nm; secondary wavelength: 405nm; measurement method: rate method

Reaction direction: negative; falling reaction temperature: 37°C; calibration mode: Linear

R1+ sample incubation time: 300 sec; reaction time: 300 sec

◆ Blank absorbance ≥1.20 (340nm)

◆ Blank change rate ≤0.003A/min

◆ Analytical sensitivity: 75.4mg/dL, △A/min≥0.010

◆ Accuracy: Relative deviation ≤10%

◆ Precision: intra-batch precision: CV≤5%

◆ Inter-batch precision: relative deviation ≤8%

◆ Linear range: 0～200mg/dL, r≥0.99