Human D-amino acid oxidase（Human DAO）

D-amino acid oxidase (DAAO, DAO, EC 1.4.3.3) is a typical flavoprotease with flavin adenine (FAD) as a prosthetic group, which oxidizes the amino group of D-amino acids to generate the corresponding keto acids and ammonia. The catalytic reaction is as follows:

 

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D-amino acid + O2 → keto acid + H2O2 + NH3

 

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D-amino acid oxidase combines with the coenzyme FAD and undergoes a reduction reaction with the D-amino acid. The D-amino acid is dehydrogenated into the corresponding imino acid and combined with the reducing FAD. This step is the only enzymatic reaction in the entire reaction; in the molecule In the presence of oxygen, reducing FAD is re-oxidized and converts oxygen into hydrogen peroxide; imino acids are hydrolyzed into A-keto acid and ammonia, but when hydrogen peroxide is present in the body, A-keto acid occurs Decarboxylation produces the corresponding acid.

 

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D-amino acid oxidase has a high degree of stereoisomer selectivity and broad spectrum for catalytic reaction substrates, and can be widely used for qualitative and quantitative analysis of D-amino acids, biosensors, and the production of L-amino acids and A-keto acids.

 

Dosage form: liquid

 

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Storage buffer: Tris buffer, pH: 8.0

 

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Vitality unit:

 

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Item number: Abt-P-5005

 

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Source: Gene recombinant expression

 

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Molecular weight: about 41 kDa (SDS-PAGE detection)

 

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Purity: ≥90% (SDS-PAGE detection)

 

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Storage conditions: 4℃ storage or -20℃ long-term storage, avoid repeated freezing and thawing

 

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Transportation conditions: low temperature ice pack

 

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Safety Tips: Not for human experimentation