Homocysteine Methyltransferase（HMT）

Homocysteine ( Hcy ) is a thiol-containing amino acid formed from methionine during S-adenosylmethionine-dependent transmethylation reactions . It has been demonstrated that even mild or moderately elevated levels of Hcy also increase the risk of atherosclerosis of the coronary, cerebral and peripheral arteries and cardiovascular disease. And currently the hcy level is regarded as the biomarker for cardiovascular disease diagnosis all over the world.

Methyltransferases are a large group of enzymes that all methylate their substrates but can be split into several subclasses based on their structural features. The most common class of methyltransferases is class I, all of which contain a Rossman fold for binding S-Adenosyl methionine (SAM). Methyltransferases can also be grouped as different types utilizing different substrates in methyl transfer reactions. These types include Protein methyltransferases, DNA methyltransferases, Natural product methyltransferases, and Non-SAM dependent methyltransferases.

SAM is the classical methyl donor for homocysteine methyltransferase (HMT ,  EC 2.1.1.10 ) , The general mechanism for methyl transfer is a SN2-like nucleophilic attack where the methionine sulfur serves as the nucleophile that transfers the methyl group to the enzyme substrate. SAM is converted to S-Adenosyl homocysteine (SAH) during this process. The breaking of the SAM-methyl bond and the formation of the substrate-methyl bond happen nearly simultaneously. These enzymatic reactions are found in many pathways and are implicated in genetic diseases, cancer, and metabolic diseases.

There are several methods for hcy level detection. And in all these methods the most time-saving, high sensitivity , high throughput is enzyme cycling methods . People has applied this method for hcy diagnosis kit, and this kit is very popular throughout the world .

The principle of the enzyme cycling methods is as the following:

Hcy:  Homocysteine                      SAM:    S-adenosyl-L-methionine HMT: Homocysteine methyltransferase        SAH:  S-adenosyl-L-homocysteine   SAHH: S-adenosyl-L-homocysteine hydrolase   ADA:  Adenosine  Deaminase

The HMT we can provide is recombinant protein, and it is of high activity and good stability, which can be used for different applications .

Product description:

Appearance: White powder, lyophilized

Preservation solution: Tris buffer, pH8.0

Basic information:

Cat#: Abt-P-Abt-P-1001

Source: Recombinant expression

pH Stability: pH6.5-8.5                  pI: 5.01

Molecular weight: About 51kDa (SDS-PAGE detection)

Purity: ≥90% (SDS-PAGE test)

Redissolution and storage: Redissolved in 30% glycerol, 4℃, store at -20℃ for long-term preservation, Avoid multiple freeze-thaw cycles.

Transportation: Cold packs 

Security: Only used for research, strictly prohibited from human or animal experiment.