TBA detection reagent

Bile acid levels in serum are important evidence reflecting liver parenchymal damage, especially in acute hepatitis, chronic active hepatitis, ethanol liver injury and cirrhosis. It is currently believed that the determination of bile acids in serum has a sensitivity of 76% and a specificity of 93% for the diagnosis of hepatobiliary diseases. In chronic liver diseases such as cirrhosis, elevated bile acids appear earlier than changes in albumin, cholesterol, cholesterol esters, and bilirubin, so it is of great significance for the diagnosis of chronic liver diseases.

Determination principle:

Bile acid is specifically oxidized by 3α-hydroxysteroid dehydrogenase (3α-HSD) and thio-oxidized nicotinamide adenine dinucleotide (Thio-NAD+) to generate 3α-ketosteroid (3α- KS) and thio-reduced nicotinamide adenine dinucleotide (Thio-NADH). In addition, the generated 3α-ketosteroids generate bile acids and oxidized coenzyme I (NAD+) in the presence of 3α-hydroxysteroid dehydrogenase (3α-HSD) and reduced coenzyme I (NADH). This cycle is repeated to amplify the trace amount of bile acid, and the absorbance change of the generated thioreduced nicotinamide adenine dinucleotide (Thio-NADH) is measured near a wavelength of 405 nm to calculate the total bile acid content.

The TBA detection reagent provided by our company has good stability and wide linear range. This reagent is suitable for fully automatic and semi-automatic biochemical analyzers or spectrophotometers that use enzymatic cycle reactions catalyzed by 3α-hydroxysteroid dehydrogenase (3α-HSD).

Features:

Liquid dual reagent, which can effectively reduce the effects of bilirubin and hemoglobin.

Strong anti-interference ability, high specificity and good stability

Reagent blank absorbance: A405nm<0.700

Linear range: 0～150μmol/L

Precision: Inter-batch relative deviation (R) ≤ 10%

Accuracy: within ±15% of the quality control product

Stability: The original packaged reagents are stored in the dark at 2 to 8°C and are valid for 12 months.