Bsu

Bsu DNA polymerase is a DNA isothermal amplification polymerase with strand displacement activity, mainly used in RPA recombinase amplification. The protein-DNA complex formed by the combination of recombinase UvsX and the primer can search for homologous sequences in double-stranded DNA; once the primer locates the homologous sequence, a strand exchange reaction will occur, and Bsu DNA polymerase starts DNA synthesis and synthesizes the template. Exponentially amplify the target region. The replaced DNA strand binds to the SSB, preventing further replacement.

We provides recombinantly expressed Bsu DNA polymerase with a molecular weight of ~68kDa, high activity and good stability. This product is a large fragment of Bsu DNA polymerase, derived from Bacillus subtilis, which is obtained by truncating the first 296 AA of the Bacillus subtilis DNA polymerase (Bsu) I gene. This enzyme retains the 5'-3' polymerase activity of Bsu I, but lacks the 5'-3' exonuclease domain. This large fragment itself lacks the 3'-5' exonuclease activity and can be used for recombination. Enzyme amplification.

Dosage form: liquid or freeze-dried powder

Storage buffer: 50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 20% glycerol, pH7.5

Molecular weight: about 68 kDa (SDS-PAGE detection)

Purity: ≥90% (SDS-PAGE detection)

Storage conditions: Store at -20°C, avoid repeated freezing and thawing.

Transportation conditions: low temperature ice pack

Thermal inactivation: 75℃, 20min

Features and applications: RPA isothermal amplification; DNA synthesis by RPA strand replacement; random primer labeling; synthesis of the second strand of cDNA; single dA tailing.

Note: Due to the lack of 3'-5' exonuclease activity, Bsu DNA Polymerase Large Fragment cannot excise 3' unpaired overhanging ends and is therefore not suitable for generating blunt ends.

     At 25°C, the large fragment of Bsu DNA polymerase retains 50% activity, which is twice that of the Klenow fragment (3'-5'exo-) at the same temperature.

Safety Tip: This reagent is only used for research and development or production, and is strictly prohibited for use in human or animal experiments.

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