GP32

gp32 protein (ssDNA binding protein), also known as T4 gp32 protein or T4 phage gp32 protein, is a single-stranded DNA (ssDNA) binding protein encoded by gene 32 of the phage T4 genome. This protein is required for T4 phage DNA replication and repair. Required, gp32 protein can be used for recombinase polymerase amplification (RPA). The protein is also widely used to stabilize and mark single-stranded DNA regions when using electron microscopy to observe intracellular DNA structures. In addition, T4 gp32 has been reported to promote restriction endonuclease and T4 DNA polymerase activities, improve reverse transcriptase efficiency during RT-PCR, and increase PCR product yield.

Dosage form: liquid or freeze-dried powder

Storage buffer: 20mM Tris-HCl, 100mM NaCl, 0.5mM DTT, 1mM EDTA, pH8.0

Molecular weight: about 35 kDa (SDS-PAGE detection)

Purity: ≥90% (SDS-PAGE detection)

Storage conditions: Store at -20°C, avoid repeated freezing and thawing.

Transportation conditions: low temperature ice pack

Thermal inactivation: 65℃, 20min

Features and applications: Stabilize or label ssDNA; increase the yield of reverse transcription products during RT-PCR; enhance DNA synthesis in vitro; used with T4 DNA Polymerase and T4 DNA Ligase for site-specific mutation experiments; When amplifying bacterial or fungal genomes from samples such as soil, humic acid can improve the yield and specificity of PCR products; it can also promote the digestion reaction of restriction endonucleases.

Safety Tip: This reagent is only used for research and development or production, and is strictly prohibited for use in human or animal experiments.