UvsY

UvsY is a bacteriophage T4 recombination regulatory protein that promotes the homologous recombination process performed by UvsX. When UvsX searches for homologous sequences, it needs to compete with single-stranded DNA-binding proteins pre-bound on single-stranded DNA for binding sites, and the UvsY protein promotes this competition and helps UvsX bind to single-stranded DNA. It enhances the DNA-dependent ATPase activity of the UvsX protein, reducing the minimum concentration required for its activity, thereby promoting strand displacement. UvsY promotes the invasion of UvsX recombinase into the ssDNA-single-stranded binding protein complex, leading to the release of the single-stranded binding protein, thus promoting the binding of UvsX to single-stranded DNA. This enzyme has no nuclease activity.

​Dosage form: liquid or freeze-dried powder

Storage buffer: 50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 20% glycerol, pH 8.0

Molecular weight: about 16 kDa (SDS-PAGE detection)

Purity: ≥90% (SDS-PAGE detection)

Storage conditions: Store at -20°C, avoid repeated freezing and thawing.

Transportation conditions: low temperature ice pack

Thermal inactivation: 60℃, 10min

Features and applications: DNA isothermal amplification; RPA amplification; gene detection.

QC:Exonuclease residue detection: When 500 U of this enzyme and 0.6 μg λ-Hind III were incubated for 16 hours at 37°C, the electrophoresis band of DNA did not change.

Endonuclease residue detection: When 500 U of this enzyme and 0.6 μg Supercoiled pBR322 DNA were incubated for 4 hours at 37°C, the electrophoresis band of the DNA did not change.

Escherichia coli DNA residue detection: 50 U The residual nucleic acid in this product was detected by E.coli 16s rDNA-specific TaqMan qPCR, and the E.coli genome residue was less than 10 copies.

Safety Tip: This reagent is only used for research and development or production, and is strictly prohibited for use in human or animal experiments.