UvsX

Recombinase polymerase amplification technology is a new technology that involves a variety of enzymes and proteins to achieve exponential amplification of nucleic acids under constant temperature conditions. It is sensitive, efficient, and cost-effective. Recombinase polymerase amplification technology generates target fragments under isothermal conditions by simulating DNA amplification in vivo. This technology mainly relies on recombinase UvsX, single-stranded binding protein gp32 and strand displacement DNA polymerase Bsu. Recombinase UvsX can unwind double-stranded DNA without heating. At the beginning of the recombinase polymerase amplification reaction, the recombinase UvsX binds the primer with the participation of ATP to form a nucleic acid-protein complex. These complexes can scan the target double-stranded DNA that is complementary to the primer sequence. Next, the nucleic acid-protein complex invades the 5' end site to form a D-shaped loop. The single-chain binding protein gp32 binds to the displaced single chain to stabilize it. At the same time, the recombinase uvsx leaves the 3' end of the oligonucleotide and is degraded and used by DNA polymerase. Afterwards, the strand-displacement DNA polymerase Bsu binds to the free 3' end of the nucleic acid-protein complex and performs chain extension to form a new complementary strand. During chain elongation, the newly synthesized single strand pairs with the original complementary strand. The above steps are repeated to achieve exponential growth of DNA.

​Dosage form: liquid or freeze-dried powder

Storage buffer: 50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, pH 8.0

Molecular weight: about 44 kDa (SDS-PAGE detection)

Purity: ≥90% (SDS-PAGE detection)

Storage conditions: Store at -20°C, avoid repeated freezing and thawing.

Transportation conditions: low temperature ice pack

Thermal inactivation: 60℃, 10min

Features and applications: DNA isothermal amplification; RPA amplification; gene detection.

QC:Exonuclease residue detection: When 500 U of this enzyme and 0.6 μg λ-Hind III were incubated for 16 hours at 37°C, the electrophoresis band of DNA did not change.

Endonuclease residue detection: When 500 U of this enzyme and 0.6 μg Supercoiled pBR322 DNA were incubated for 4 hours at 37°C, the electrophoresis band of the DNA did not change.

Escherichia coli DNA residue detection: 50 U The residual nucleic acid in this product was detected by E.coli 16s rDNA-specific TaqMan qPCR, and the E.coli genome residue was less than 10 copies.

Safety Tip: This reagent is only used for research and development or production, and is strictly prohibited for use in human or animal experiments.

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