CRISPR-Cas12a（Cpf1）

CRISPR genome editing technology has developed rapidly since it was reported and has been widely used in genome editing of mammalian cells, showing great development potential in the treatment of genetic diseases and genetic testing. CRISPR Cas nucleases mainly include two types: one is Cas13a and Cas13b that can cleave RNA strands under RNA guidance; the other is Cas12a and Cas14 that can cleave DNA strands under RNA guidance. Cas12a and Cas13 both have a common feature. In addition to cutting the target DNA or RNA sequence, they can also cut other DNA or RNA sequences, but this additional cutting ability of the Cas13 enzyme is stronger than that of Cas12a. The target genes of Cas12a are ssDNA and dsDNA, and the Cas12a guide chain needs to recognize the PAM sequence (the PAM sequence of Cas12a is TTTV).We provides genetically recombinant cas12a nuclease with high activity and good stability.

Source: Gene recombinant expression

Molecular weight: about 180 kDa (SDS-PAGE detection)

Purity: ≥90% (SDS-PAGE detection)

Storage conditions: -20℃ (it is recommended to store in separate packages to avoid repeated freezing and thawing that affects protein activity)

Transport conditions: low temperature ice pack

Safety Tips: Not for human experimentation

   Recommended dosage: Add approximately 5 pmol to 50 μL reaction system.

Constant temperature amplification (RPA) combined with cas12a gene editing produces fluorescent signals (left is negative control):