P450scc，CYP11A1

In mammals, the synthesis of pregnenolone from cholesterol is the first and rate-limiting step in the synthesis of sterol hormones. This reaction is carried out in the mitochondria of the adrenal cortex and is catalyzed by cytochrome CYP11A1 (also called P450scc), which also works with Adx and AdR. CYP11A1, Adx and AdR belong to the cholesterol hydroxylase/lyase system (CH/L), which catalyzes the initial step of steroid synthesis in mammals, that is, the process of producing pregnenolone from cholesterol. Pregnenolone is an important precursor of steroid hormones. The reaction process includes three consecutive monooxygenation reactions, namely the generation of 22R-hydroxycholesterol (22HC), the generation of 20R, 22R-dihydroxycholesterol, and the cleavage of the C20-C22 bond. Each single oxygenation reaction requires the participation of two electrons and one molecular oxygen. Electrons are provided by NADPH and passed to P450scc through NADPH-AdR and Adx. Adx forms a complex with P450scc and acts as a mobile electron transporter.

Dosage form: freeze-dried powder, or liquid

Storage buffer: 20 mM Tris-Cl, pH8.0

Enzyme activity: 600-700 U/mg

Activity unit: Unit of CYP11A1 enzyme catalytic activity: At 37°C, under specific reaction conditions, the amount of enzyme required to generate 1 nmol of the product pregnenolone within 1 hour using cholesterol as a substrate is defined as 1 U.

Source: Gene recombinant expression

Molecular weight: about 60 kDa (SDS-PAGE detection)

Purity: ≥90% (SDS-PAGE detection)

Storage conditions: The solution is packaged at -80 degrees and stored for a long time. Avoid repeated freezing and thawing

Transportation conditions: low temperature ice pack

Safety Tips: Strictly prohibited for use in human experiments