Carboxypeptidase B（CPB）

Carboxypeptidase B (EC 3.4.17.2), also well known as protaminase, pancreatic procarboxy-peptidase B (PCPB), tissue carboxypeptidase B, peptidyl-L-lysine (L-arginine) hydrolase is a highly pancreas-specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those exopeptidases that cleave off basic C-terminal residues, CPB1specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of CPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain.The secreted CPB zymogen is converted to enzymatically active CPB by limited proteolysis by trypsin.

During the industrial production of insulin, the CPB is necessary.

The CPB we provide is recombinant protein , and it is of high activity and stability, which can be used for different applications .

Product description:

Appearance: White powder, lyophilized

Preservation solution: 20mM NaAc, pH3.5

Specific activity: ≥180U/mg

Unit Definition: One unit will catalyze 1.0 μmole of Hip-L-Arg per min at pH 7.65 at 25°C.

Basic information:

Cat#:Abt-P-5001                          

Source: Recombinant expression

Molecular weight: About35kDa (SDS-PAGE detection)

Purity: ≥90% (SDS-PAGE test)

Redissolution and storage: Redissolved in 20% glycerol, 4℃, store at -20℃ for long-term preservation, Avoid multiple freeze-thaw cycles.

Transportation: Cold packs 

Security: Only used for research, strictly prohibited from human or animal experiment.