Adenosine Deaminase（ADA）

Adenosine deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme (EC 3.5.4.4) involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues.

Present in virtually all mammalian cells, its primary function in humans is the development and maintenance of the immune system. However, the full physiological role of ADA is not yet completely understood.

ADA irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the substitution of the amino group for a hydroxyl group. 

Inosine can then be deribosylated (removed from ribose ) by another enzyme called purine nucleoside phosphorylase (PNP), converting it to  Hypoxanthine.

Product description:

Appearance: White powder ,  lyophilized

Preservation solution: Tris buffer, pH8.0

Specific activity：150 - 200U/mg

Unit Definition： One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25°C.

Basic information:

Cat#: Abt-P-1003                          

Source: Recombinant expression

pH Stability: pH7.0-9.0                  pI: 5.43

Molecular weight: About 53kDa (SDS-PAGE detection)

Purity: ≥90% (SDS-PAGE test)

Redissolution and storage: Redissolved in 20% glycerol, 4℃, store at -20℃ for long-term preservation, Avoid multiple freeze-thaw cycles.

Transportation: Cold packs 

Security: Only used for research, strictly prohibited from human or animal experiment