Vascular Endothelial Growth Factor（VEGF165）

Vascular endothelial growth factor (VEGF) is also known as vascular permeability factor (VPF), or VEGF-A.

 

 

 

VEGF-A is a dimeric glycoprotein composed of two identical subunits (Mr 23000) cross-linked by disulfide bonds. The molecular weight is 40-45KD. Its gene is located on chromosome 6p21.3, with a full length of 14kb and consists of 8 exons. It consists of sub-sub and 7 introns. Due to different splicing methods of its mRNA, 7 existing forms are formed, namely VEGF121, VEGF145, VEGF148, VEGF183, VEGF165, VEGF189, and VEGF206, each containing 121-206 amino acids and connected by disulfide bonds to form homodimers. VEGF165 has 44 amino acids encoded by exon 7 and has a high affinity for heparin.

 

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VEGF121, VEGF145, and VEGF165 can all induce endothelial cell proliferation and new blood vessel formation in vivo. Most cells expressing VEGF-A can produce several VEGF-A variants at the same time, with VEGF121 and VEGF165 being the most common, followed by VEGF189.

 

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Each variant of VEGF-A (VEGF121~206) has different binding abilities with heparin and heparin sulfate. Heparin or heparin sulfate can enhance the affinity of VEGF-A and its receptor.

 

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Because VEGF165 has the highest abundance in cDNA amplification, it is the most widely used in clinical and animal experiments. VEGF165 is expressed in a variety of cells, including endothelial cells, macrophages, and T cells. VEGF165 participates in angiogenesis, vascular endothelial cell growth, migration, penetration, etc.

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Human VEGF165 (his tag)

 

Cat#Abt-P-4025

 

Expression host: HEK293

 

​Molecular weight: 22-25 kDa

 

Concentration: 1-2mg/mL

 

Purity: >90%

 

Storage environment: 1×PBS (pH: 7.4)

 

Storage conditions: short-term storage at 4℃, long-term storage at -20℃. Avoid repeated freezing and thawing

 

Safety Tips: Not for human experimentation