TNFα （ human tumor necrosis factor, human TNFα）

Tumor necrosis factor (TNF) is mainly produced by activated macrophages, NK cells and T lymphocytes. In 1985, Shalaby named the TNF produced by macrophages as TNF-α, and the lymphotoxin (lymphotoxin, LT) produced by T lymphocytes as TNF-β. Although TNF-α and TNF-β are only about 30% homologous, they share a common receptor. The biological activity of TNFα accounts for 70% to 95% of the total activity of TNF, so the commonly used TNF currently refers to TNF-α.

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The human TNF-α precursor is composed of 233 amino acids (26kDa), which contains a signal peptide composed of 76 amino acid residues. Under the action of TNF converting enzyme TACE, the signal peptide is removed to form a mature 157 amino acid residues. TNF-α (17 kDa). Since there is no methionine residue, there is no glycosylation site, and the two cysteines at positions 69 and 101 form an intramolecular disulfide bond. Human TNF-α has 79% amino acid composition homology with mouse TNF-α, and the biological effects of TNF-α do not appear to be significantly species-specific.

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The human TNFα provided by our company has high purity and good activity.

Human TNFα(His tag)

Cat#Abt-P-4023

Expression host: HEK293

Protein sequence number: NP_000585.2 (extracellular region)

​Molecular weight: 17 kD

Concentration: 1-2mg/mL

Purity: >90%

Storage environment: 1×PBS (pH7.4)

Storage conditions: short-term storage at 4℃, long-term storage at -20℃. Avoid repeated freezing and thawing

Safety Tips: Not for human experimentation